EzRIPA buffer is one of the most reliable buffers for the lysis of cultured mammalian cells and tissue samples. The kit includes protease and phosphatase inhibitors for the prevention of proteolysis and further phosphorylation of proteins during extraction. The buffer enables protein extraction from cellular fractions that are compatible with many applications such as immunoassays, enzyme activity. Additionally, the RIPA buffer is an amine-free buffer making it ideal for downstream applications like protein conjugation to dyes or biotin.