ATTO's Subcellular Fractionation Kit Separates and Extracts Cytosol, Nuclei, Membrane, and Insoluble Proteins from Mammalian Cells. This kit is idea for sample preparation for electrophoresis, immunoprecipitation, ELISA, chromatin immunoprecipitation assay, enzyme activity experiments, and more. Simply centrifuge after adding each of the four extraction buffers. No cross contamination of cellular fractions. Collect all cellular fractions in 3 hours. Ideal for mammalian tissue or frozen cells. Ideal for mammalian tissue and frozen cells.