Factor IX

Factor IX (FIX, Christmas Factor) is a vitamin K-dependent\nglycoprotein produced in the liver. Plasma concentration of FIX\nis normally around 5ug/ml (87nM) in plasma. The biological\nimportance of FIX is demonstrated in Haemophilia B\n(Christmas disease). This is an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in FIX function. In its proenzyme or zymogen form, FIX is a single chain molecule of 55kD. It contains two EGF-like domains and an amino-terminal domain containing 12-g-carboxy-glutamic acid (Gla) residues. These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions. \n\nApplications:\nSuitable for use as ELISA, Neutralization and other solid phase immunoassays for Factor IX and its activation products. Other applications not tested.\n\nRecommended Dilutions:\nELISA: Suitable for use as the capture antibody.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0017-08

Size

500ug

Applications

ELISA

Hosts

Sheep

Reactivities

Hum, Prc

Form

Supplied as a liquid in 10mM HEPES, pH7.4, 0.15M sodium chloride, 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Lawson, J.H., Mann, K.G., Cooperative Activation of Human FIX by the Human Extrinsic Pathway of Coagulation. JBC 266: 11317-11327 (1991). 2. Enfield, D.L,, Thompson, A.R., Cleavage and Activation of Factor IX by Serine Proteases. Blood 64: 821-831 (1984). 3. Limentani, S.A., Furie, B.C., Furie, B., in Hemostasis and Thrombosis Thrombosis, 3rd Edition, eds. Colman,R.W., Hirsh, J., Marder, V.J., Salzman, E.W., pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA (1994).

Additional Info

Recognizes human FIX as analyzed by immunoelectrophoresis and ELISA. Species crossreactivity: porcine.