Factor IX (HRP)

Factor IX (FIX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FIX is normally around 5ug/ml (87nM) in plasma. The biological importance of FIX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in FIX function. In its proenzyme or zymogen form FIX is a single chain molecule of 55kD. It contains two EGF-like domains and an amino-terminal domain containing 12 g-carboxy-glutamic acid (Gla) residues. These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of FIX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146-Ala147 and between Arg180-Val181. The terminal activated product in either case is F.IXab, a two-chain enzyme consisting of a heavy chain (28kD), a light chain (18kD) and an activation peptide product of 11kD. FIX can also be cleaved into inactive products by thrombin and by elastase. The activity of FIXab in plasma is inhibited by antithrombin and this inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin (1-3).\n\nApplication:\nSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

F0017-07B

Size

100ug

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with HRP. Rz ratio (Reinheitszahl, A403/A280): 0.461, as determined spectrophotometrically.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Lawson, J.H., Mann, K.G., (1991) J. Biol. Chem. 266:11317-11327. 2. Enfield, D.L., (1984) Blood 64:821-831. 3. Limentani, S.A., et al., in Hemostasis and Thrombosis Thrombosis, 3rd Edition, eds. R.W. Colman, J. Hirsh, V.J. Marder, E.W. Salzman, pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA (1994).

Additional Info

Recognizes human Factor IX as demonstrated by by ELISA and Immunoelectrophoresis.