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Factor IXa DEGR, Bovine

Cat no: F0017-18

Factor IXa DEGR, Bovine

Factor IXa with the active site irreversibly blocked by the fluorescent inhibitor, Dansyl-EGRck.\nFactor IXa is also available with the active site irreversibly blocked by the tripeptide chloromethyl ketone, EGRck. \n\nActivation of Factor X By Factor IXa: The intrinsic factor Xase complex consists of an enzyme, factor IXa, and a cofactor, factor VIIIa, assembled on a phospholipid surface in the presence of calcium ions. The enzyme complex proteolytically cleaves a 10,000 molecular weight activation peptide from the NH2-terminal of the heavy chain of factor X, thus expressing the active-site of factor Xa.\n

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SPECIFICATIONS

Catalog Number

F0017-18

Size

100ug

Form

Supplied as a liquid in 50% glycerol/H2O.

Purity

(same/more than)95%. Factor IXa is prepared from highly purified factor IX by activation with factor XIa, as described by Lindquist et al. (5). The factor IXa is further purified by gel filtration, followed by immunoaffinity purification. Purity is assessed by SDS-PAGE analysis. Activity is determined in a one-stage clotting assay.

References

1. Thompson, A.R., Blood, 67, 565 (1986).\n2. Hedner, U., et al., In Hemostasis and Thrombosis, 2nd edition, ed. R.W. Colman, J. Hirsh, V.J. Marder, E.W. Salzman, pp., 39-47. J.P. Lippincott Co, Philadelphia, 1987.\n3. Discipio, R.G., et al., Biochemistry, 16, 698 (1977).\n4. McMullen, B.A., et al., BBRC, 115, 8 (1983).\n5. Lindquist, P.A., et al., J. Biol. Chem., 253, 1902 (1978).

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