Factor VII (F.VII, Stable Factor, Proconvertin)

Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444aa precursor. After cleavage of a signal peptide (residues 1-38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444). \n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1ug/ml detected recombinant human Factor VII.\nImmunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Factor VII.\nImmunocytochemistry: 8-25ug/ml using immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs).\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNS0 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F0015-02

Size

100ug

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human Factor VII. Does not crossreact with recombinant human Kallikrein-1, -2, -3, -4, -5, -7, -8, -9, -10, -11, -12, -13, -14, -15, -B1, recombinant human HGFA, recombinant human Factor X, recombinant human Factor XI, recombinant human Thrombin, recombinant human uPA-1 or recombinant human PROC.