We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Although not required, proteolytic treatment of formalin fixed paraffin embedded tissue sections may enhance the immunostaining in overfixed tissues. Specificity: This antibody reacts with human factor VIII related antigen. It stains endothelial cells and megakaryocytes in various human tissues. This antibody does not cross-react with any other cell types. Cellular Localization: Cytoplasmic.
Applications:
Suitable for use in Immunohistochemistry . Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
Human tonsil
Cellular Localization:
Cytoplasmic
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.