Factor XIII subunit A (HRP)

Factor XIII (F.XIII, fibrin stabilizing factor) is the proenzyme form of a transamidase that is essential for normal haemostasis and\nfibrinolysis, wound healing, female fertility and foetal development. Extracellular F.XIII consists of A subunits (83kD each) which contain the enzyme moiety, and B subunits (76kD each) which act as a carrier protein for the A subunit in circulation. Both subunits are produced under separate genetic control. In plasma, F.XIII exists as a non-covalent tetrameric complex (320kD) of two A-subunits and two B-subunits (A2B2). The concentration of F.XIII tetramer in plasma is 25ug/ml (80nM). An intracellular form of F.XIII is found in\nplatelets, megakaryocytes and monocytes. This form of F.XIII\npresents as a dimer of two A-subunits only and has a molecular\nweight of160 kD. The importance of these intracellular stores is\ndemonstrated by the observation that platelets can contribute up to half of the F.XIII activity in platelet rich plasma. The activation of F.XIII involves several steps. Thrombin cleaves after Arg37 of each Asubunit in the A2B2 tetramer, releasing a 4.5kD activation peptide. Additional conformational changes induced by the binding of calcium, and by dissociation of the B-subunits from the A-subunit dimer are required to obtain full enzyme activity. F.XIIIa is a cysteine protease that catalyses the formation of gamma-glutamyl-epsilon-lysyl bonds\nbetween the gamma and alpha chains of polymerised fibrin molecules. Other proteins found crosslinked into fibrin clots by F.XIIIa include fibrinogen, alpha2antiplasmin, fibronectin, vitronectin and von Willebrand factor(1-3).\n\nApplications: \nSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-46B

Size

200ug

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. No preservatives are added. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. McDonagh J; Structure and Function of Factor XIII; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp301-313, J.B. Lippincott Co., Phiadelphia PA, USA, 1994. 2. Inbal A, Muszbek L; Coagulation Factor Deficiencies and Pregnancy Loss; Seminars in Thrombosis and Haemostasis 29, pp171-172, 2003. 3. Murdock PJ, Owens DL, Chitolie A, Hutton RA, Lee CA; Development and Evaluation of ELISAs for Factor XIIIA and XIIIB Subunits in Plasma; Thrombosis Research 67 pp73-79, 1992

Additional Info

Recognizes human Factor XIII subunit A.