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Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA,  Azide & Glycerol Free)

Cat no: F0019-50GX

Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA, Azide & Glycerol Free)

Factor XIII is a beta-globulin found in plasma and is composed of two subunits. Factor XIIIa is the catalytic subunit and is a dimer of MW 160kD. Factor XIII is present in plasma as an a2b2 heterodimer (MW 320kD). Whereas in platelets, only the a2 unit exists. Factor XIIIa is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 5-10ug/ml for 2hrs at RT.\nImmunohistochemistry (Formalin/paraffin): 10-20ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman recombinant Factor XIIIa. Capillary hemangioma, dermatofibroma and placenta. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-50GX

Size

500ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives and no stabilizing proteins added. Also available with BSA and azide. See F0019-50G.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Factor XIIIa, both reduced and non-reduced forms. Does not crossreact with human Factor XIII B-chain or human Factor XII.

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