FADD (Fas-Associated Death Domain Protein, MORT1)

Fas-associating death domain containing protein (FADD)/MORT1 is a 23kD cytosolic protein. It has an apparent molecular mass of 28kD on SDS PAGE. It serves as a signal transducer of Fas-induced apoptosis (1, 2). FADD contains a death domain homologous to that of Fas. It interacts with the cytoplasmic death domain of Fas in order to initiate the apoptotic signalling cascade. Through its amino-terminal death effector domain (DED), FADD binds to cytosolic proteins such as TRADD, a cytosolic death domain-containing protein that asssociates with the cytosolic portion of TNF receptor, or MACH/FLICE/caspase-8, a protease with sequence homology to ICE-CED-3 protease family, in order to transduce the death signal (3, 4). The FADD-FLICE containing signalling complex is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, which inhibits the protease activity of FLICE, and the short and long form of FLIP, which bind to FADD-FLICE complex (2, 5).\n \nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 1-4ug/ml. Detects 200ug heat shocked HeLa cell lysate.\nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nHeLa Heat Shocked Cell Lysate, Mouse Brain Tissue Extract, \nRat Brain and Tissue Extract.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-51B

Size

50ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in borate buffered saline, pH 8.2, [25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt)], before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chinnaiyan, A.M., et al., Cell 81: 505-512 (1995). 2. Irmler, M., et al., Nature 388: 190-195 (1997). 3. Varfolomeev, E.E., et al., J. Exp. Med. 183: 1271-1275 (1996). 4. Boldin, M.P., et al., Cell 85: 803-815 (1996). 5. Enari, M., et al., Nature 375: 78-81 (1995). 6. Besnault, L., et al., J. Immunol. 167(2): 733-740 (2001).

Additional Info

Detects a ~30kD protein, corresponding to the apparent molecular mass of FADD on SDS-PAGE Immunoblots. Antibody specificity is confirmed by peptide inhibition studies. Species Crossreactivity: Human, monkey, mouse and rat.