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FADD (Fas-Associated Death Domain Protein, MORT1)

Cat no: F0019-51F


Supplier: United States Biological
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Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1beta-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.1-6 Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Hybridoma: Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FADD protein. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0019-51F
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 100ul
Form: Supplied as a liquid inPBS pH 7.2, 0.1% sodium azide.
P type: Mab
Isotype: IgG2b,k
Purity: Purified by immunoaffinity chromatography.
References: 1. Chinnaiyan, A.M., K. O
Additional info: Recognizes human FADD.

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