FAIM (Fas Apoptotic Inhibitory Molecule)

The susceptibility of primary splenic B cells to Fas-mediated apoptosis is regulated in a receptor-specific fashion (reviewed in 1). Terminal effectors of B cell Fas-resistance include the known anti-apoptotic proteins Bcl-xL, FLIP, and a recently identified protein termed FAIM (2). This molecule is broadly expressed in various tissues and exists in at least three isoforms. It is thought that resistance to Fas killing via increased expression of FAIM protects foreign antigen-specific B cells during interactions with FasL-bearing T cells whereas autoreactive B cells are deleted via Fas-dependent cytotoxicity. More recent results have indicated that FAIM interacts with both Trk and p75 neurotrophin receptor and may play a role in promoting neurite outgrowth in different neuronal systems by a mechanism involving the activation of NF-kB and the Ras-ERK pathway (3). \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 5-10ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman spleen tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-53J

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Rothstein TL. Inducible resistance to Fas-mediated apoptosis in B cells. Cell Res. 10:245-66. (2000).2. Schneider TJ, Fischer GM, Donohoe TJ, et al. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. J. Exp. Med. 189:949-55 (1999).3. Sole C, Dolcet X, Segura MF, et al. The death receptor antagonist FAIM promotes neurite outgrowth by a mechanism that depends on ERK and NF-kappa B signaling. J. Cell Biol. ; 167:479-92 (2004).

Additional Info

Recognizes human FAIM. Species Crossreactivity: Mouse.