FAIM1 (Fas Apoptotic Inhibitory Molecule 1, FAIM)

FAIM (Fas apoptosis inhibitory molecule) was identified as a protein that was inducibly expressed in B lymphocytes resistant to Fas-mediated apoptosis (1). Expression of FAIM inhibits receptor-mediated apoptosis in B cells as well as other cell types (1-3). FAIM is expressed in germinal center B cells, is positively regulated by IRF-4, and is also capable of inducing IRF-4 expression in a feed-forward mechanism (4). FAIM also regulates T cell receptor-mediated apoptosis by modulating Akt activation and Nur77 expression (2). Knockout mice for FAIM show an increased sensitivity to Fas-mediated apoptosis within B and T cells as well as hepatocytes (5). An alternatively spliced form of FAIM, termed FAIM-L, is found predominantly in the brain (6). In the nervous system, the originally identified FAIM does not appear to play a role in apoptosis, but rather can promote neurite outgrowth through the activation of Erk and NF-kB pathways (7). In contrast, FAIM-L does inhibit neuronal cell death triggered by death receptors (3).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0019-53J3

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1.Schneider, T.J. et al. (1999) J Exp Med 189, 949-56. 2.Huo, J. et al. (2010) J Biol Chem 285, 11827-35. 3.Segura, M.F. et al. (2007) J Neurosci 27, 11228-41. 4.Kaku, H. and Rothstein, T.L. (2009) J Immunol 183, 5575-81. 5.Huo, J. et al. (2009) Cell Death Differ 16, 1062-70. 6.Zhong, X. et al. (2001) Mol Immunol 38, 65-72. 7.Sole, C. et al. (2004) J Cell Biol 167, 479-92.

Additional Info

Recognizes endogenous levels of total human FAIM.