FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb 2 adapter protein and c Src PTK with FAK in vivo, and also results in activation of the ERK 2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb 2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb 2 SH2 domain binds directly to tyrosine phosphorylated FAK, and the binding site has been identified as Tyr 925 by site directed mutagenesis.\n\nCellular Localization: Cytoplasmic and focal contacts.\n\nPositive Control: HUVEC, SW156 or IMR-5 cells.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F0019-55A7

Size

500ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, Xen/Amph

Form

10mM PBS, pH7.4, 0.2%BSA

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Hanks SK & Polte TR Signaling through focal adhesion kinase. Bioessays 19:137-45 (1997). <PUBMED:9046243>Ilic D et al. Focal adhesion kinase: at the crossroads of signal transduction. J Cell Sci 110 (Pt 4):401-7 (1997). <PUBMED:9067592>Owens LV et al. Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors. Cancer Res 55:2752-5 (1995). <PUBMED:7796399>

Additional Info

Species Crossreactivity: Crossreacts with canine, Human, Mouse, Rat and Xenopus laevis.Not yet tested in other species.

Alternative Names

Focal Adhesion Kinase, FADK 1, pp125FAK, Protein Tyrosine Kinase 2