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FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Cat no: F0019-55A7

Supplier: United States Biological
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FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb 2 adapter protein and c Src PTK with FAK in vivo, and also results in activation of the ERK 2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb 2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb 2 SH2 domain binds directly to tyrosine phosphorylated FAK, and the binding site has been identified as Tyr 925 by site directed mutagenesis. Cellular Localization: Cytoplasmic and focal contacts. Positive Control: HUVEC, SW156 or IMR-5 cells. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0019-55A7
Reactivities: Human, Mouse, Rat, Canine, Xenopus/Amphibian
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 500ul
Form: 10mM PBS, pH7.4, 0.2%BSA
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
Alternative names: Focal Adhesion Kinase, FADK 1, pp125FAK, Protein Tyrosine Kinase 2
References: Hanks SK & Polte TR Signaling through focal adhesion kinase. Bioessays 19:137-45 (1997). Ilic D et al. Focal adhesion kinase: at the crossroads of signal transduction. J Cell Sci 110 (Pt 4):401-7 (1997). Owens LV et al. Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors. Cancer Res 55:2752-5 (1995).
Additional info: Species Crossreactivity: Crossreacts with canine, Human, Mouse, Rat and Xenopus laevis.Not yet tested in other species.

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