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FAK, phosphorylated (Tyr861) (Focal Adhesion Protein Tyrosine Kinase)

Cat no: F0019-56M

FAK, phosphorylated (Tyr861) (Focal Adhesion Protein Tyrosine Kinase)

Applications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: A 1:1000. detected phospho-FAK (Tyr861) in pervanadate treated Huvec lysates. Lysates from Huvec treated with pervanadate were resolved by electrophoresis, transferred to PVDF and probed with F0019-56M for 2 hours at RT. Optimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-56M

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Ch/Bird

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ohtaki, T., et al., Nature 411: 613-616, 2001. 2. Zabe, M., et al., J. Biol. Chem. 276: 14704-14709, 2001. 3. Gervais, F.G., et al., J. Biol. Chem. 273: 17102-17108, 1998.

Additional Info

Recognizes human phosphorylated FAK (Tyr861), Mr 125kD. Species Crossreactivity: mouse and chicken. \nSpecies Sequence Homology: rat and frog.

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