Fas Ligand (FASL) (BSA Free)

Supplier:

United States Biological

Cat no: F0019-65H1

Fas Ligand (FASL) (BSA Free)

Applications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000(ECL). We recommend the use of 0.5-1% milk in diluents used for primary antibody and secondary antibody-enzyme conjugates to suppress background. Rat, mouse and human FASL have 278, 279, and 281aa residues, respectively (Calculated MW of ~32kD). Western blot of the oocyte has detected a specific 31-kD band (1). A 40kD FASL has been detected in mouse T cells (1). An alternate detecting reagent, mFAS-Fc (1) also detected a cell surface protein of about 40kD.\n\nELISA: 0.5-1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nF0019-65R\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-65H1

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 100mM Tris, pH 7.5. No stabilizing proteins added. Also available with BSA. See F0019-65H.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

(1) Mita E et al (1994) BBRC 204, 468-474; 2. Hakuno N et al (1996) Endocrinology 137, 1938-48; 3. Hahne M (1995) Intl. Immunol. 7, 1381-1386; 4. Suda T and Nagata S (1994) J Exp. Med. 179, 873-879; 5. Takahashi T et al (1994) Cell 76, 969-976

Additional Info

Recognizes human FASL. Species sequence homology: mouse and rat-92%.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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