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Fas Ligand (FASL)

Cat no: F0019-65B

Fas Ligand (FASL)

The FAS ligand (FASL, CD95), a member of the tumor necrosis factor family, induces apoptosis in FAS-bearing cells (1). FASL is a type II membrane receptor with a soluble form that can be released into the extracellular fluid by proteolytic processing. Various cells express Fas, whereas FASL is expressed in activated splenocytes and thymocytes (2). In the immune system, Fas and FasL are involved in down-regulation of immune reactions as well as in T cell-mediated cytotoxicity. Malfunction of the Fas system causes lymphoproliferative disorders and accelerates autoimmune diseases, whereas its exacerbation may cause tissue destruction.\n\nApplications: \nSuitable for use in Flow Cytometry and ELISA. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1.2ug labels 10e6 cells. BOSC23 cells were transiently transfected with an expression vector encoding either FasL or an irrelevant protein (control transfectant). Binding of FASL was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FasL transfected cells.\nELISA: 1:200-1:400\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F0019-65B

Size

100ug

Applications

ELISA, FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes the human Fas Ligand (FASL). Reacts with the complete native protein expressed on transfected mammalian cells.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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