FASN Human, Control Peptide (Fatty Acid Synthase)

Supplier:

United States Biological

Cat no: F0020-12

FASN Human, Control Peptide (Fatty Acid Synthase)

Fatty Acid Synthase (FASN) catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer\ncell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FASN is fused in-frame with the C-terminus of ER-alpha. FASN is highly expressed in adipocytes and liver. FASN is a central enzyme in de novo lipogenesis. FASN is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. FASN: Bovine: 2513 aa; Pig: 2411 aa; Chick: 2511 aa; Mouse: 2504 aa; Rat: 2505 aa; Human: 2511 aa; 273.4kD; 17q25. Expression: Ubiquitous; more prominent expression in brain, lungs, and liver.\n\nSource:\nSynthetic peptide corresponding to 18aa of ~N-termial of human FASN (KLH) (P49327). \n \nApplications: \nSuitable for use in ELISA and Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng.\nAntibody Blocking: 5-10ug/1ug of Affinity Purified antibody or 1ul of serum.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0020-12

Size

100ug

Applications

ELISA

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Prc

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Purity

Purified.

References

1. Jayakumar A. et al., (1995). Proc Natl Acad Sci USA. 12;92(19):8695-9.

Additional Info

Recognizes human FASN. Species Crossreactivity: mouse, rat bovine-100%; pig-94%; and chick 83%.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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