Fatty Acid Amide Hydrolase (FAAH)

Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis. Cannabis affects cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoid, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2. Neurons and astrocytes have been found to re-uptake and hydrolyse anandamide rapidly, resulting in the formation of arachidonic acid and ethanolamine. The uptake mechanism has been shown to be mediated by a saturable, selective, temperature-dependent and Na+-independent transporter. Anandamide hydrolysis is catalyzed by a membrane-bound amidohydrolase (called anandamide amidohydrolase or fatty acid amide hydrolase, FAAH). FAAH (rat/mouse/human 579 aa; chromosome 1p34-p35; mol wt ~67kD) sequence analyses suggest a single predicted transmembrane domain at the extreme N-terminus of the enzyme. Distribution of FAAH parallels CB1 in rat brain suggesting that FAAH participates in cannabinoid signalling mechanisms. The sn-2-Arachidonylglycerol (2-AG), initially isolated from intestine, appears to be the second endogenous CB ligand (CB1 Ki = 472 nM; CB2 Ki = 1400 nM). 2-AG concentration in the brain is 170 times greater than anandamide. FAAH hydrolyzes 2-AG at a rate four times faster than that for anandamide hydrolysis.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 \nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-68B

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, with 0.05% sodium azide and 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

Patricelli MR et al (1998) Biochemistry 37, 15177-15187; Maccarone M et al (1998) J. Biol. Chem. 273, 32332-32339; Cravatt BF et al (1996) Nature 384, 83-87; Giang Dk et al (1997) PNAS 94, 2238-2242; Thomas EA et al (1997) J. Neurosci. 50, 1047-1052; Wan M et al (1998) Genomics 54, 408-414

Additional Info

Recognizes human Fatty Acid Amide Hydrolase (FAAH).