Fatty Acid Binding Protein, Heart (FABP) (Biotin)

Supplier:

United States Biological

Cat no: F0019-75L

Fatty Acid Binding Protein, Heart (FABP) (Biotin)

FABP is a plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 2 hours after AMI and return to normal generally within 18 to 24 hours. The concentration of FABP in the skeletal muscle is 20 times lower than in cardiac tissue (for myoglobin the same content for cardiac and skeletal tissue), making FABP cardiac-specific cf. myoglobin. This makes FABP a useful biochemical marker for the early assessment or exclusion of AMI. FABP also appears a useful plasma marker for the estimation of myocardial infarct size.\n\nApplications:\nAnti-human heart FABP is useful for immunoassays, Immunoprecipitation, Immunohistology of frozen sections, Western Blots and for purification.\n\nRecommended Dilution:\nImmunohistology: 1:10\nWestern Blot: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-75L

Size

50ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Conjugates

Biotin

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA. Conjugated to Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149.

Additional Info

Recognizes human heart FABP. Shows no binding to human intestinal or liver FABP. Stains heart muscle cells and striated skeletal muscle cells in immunohistology. Can be used to detect ischemia areas of human heart. Crossreactive with rat and mouse H-FABP.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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