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FBG3 (F-box 3)

Cat no: F0019-98F


Supplier: United States Biological
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F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Positive control: Recombinant human FBG3. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F0019-98F
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4. No preservative added.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Ilyin, G.P., et al., (2002), "A new subfamily of structurally related human F-box proteins", Gene, 296(1-2): 11-20.
Additional info: Recognizes human FBG3 (aa103-119). Sequence homology: mouse, rat (94%).

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