FCRLB, aa18-427, Recombinant, Mouse (FCRL2, FCRY, FREB2, Fc Receptor-like B, Fc Receptor-like and Mucin-like Protein 2, Fc Receptor-like Protein 2, Fc Receptor-related Protein Y)

FCRLB, is a 60kD protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRLB binds to purified mouse IgG. Mouse FCRLB shares 82%, 94% aa sequence identity with human and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues (1-3). When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization (4, 5). FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center (4, 5). It is up-regulated in B cells by BAFF and LPS (3). Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma (3, 4, 6). It is preferentially expressed in non-proliferating cells and at the onset of apoptosis (3, 5).\n\nSource: \nRecombinant corresponding to aa18-427 from mouse FCRLB, fused with 6-His tag at C-terminal, expressed in myeloma cell line, NSO.\n\nMolecular Weight: \n~46.5kD\n\nEndotoxin Level:\n(same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by its ability to bind mouse IgG with an estimated kD<300nm.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0025-73A

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

Purity

~80% (SDS-PAGE)

References

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525. 2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431. 3. Masuda, K. et al. (2005) Gene 363:32. 4. Chikaev, N.A. et al. (2005) Genomics 85:264. 5. Wilson, T.J. and M. Colonna (2005) Genes Immun. 6:341. 6. Inozume, T. et al. (2005) Int. J. Cancer 114:283.