FDXR (NADPH:adrenodoxin Oxidoreductase, Mitochondrial, AR, Adrenodoxin Reductase, Ferredoxin-NADP(+) Reductase, Ferredoxin Reductase, ADXR)

Ferredoxin reductase is a ubiquitous flavoenzyme, containing noncovalently bound FAD as a prosthetic group. It plays a role in delivering NADPH or low potential one-electron donors such as ferredoxin and flavodoxin to redox-based metabolisms in plastids, mitochondria and bacteria. In mammals, ferredoxin reductase is loosely associated with the inner mitochondrial membrane and receives electrons from NADPH. These electrons are transferred to ferredoxin which shuttles electrons to cytochrome P450 in the adrenal cortex mitochondrial steroid hydroxylation systems. Function serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:3000\nImmunoprecipitation: 1ul\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cells\n\nCellular Localization:\nMitochondrion matrix\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F3981-01A

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Carrillo, N., and Ceccarelli, E. D. (2003) Eur. J. Biochem. 270, 1900-1915. 2. Lambeth, J. D. and Kamin, H. (1977) J. Biol. Chem. 252, 2908-2917. 3. Lin, D. et al. (1990) Proc. Natl. Acad. Sci. USA. 87,8516-8520.

Additional Info

Recognizes human Ferredoxin Reductase. Species Crossreactivity: Mouse and rat.