Ferredoxin-1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and it is involved in the synthesis of bile acid and vitamin D. Ferredoxin-1 has been identified in a number of different tissues, but all forms have been shown to be identical and not tissue specific.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-10,000
Immunocytochemistry: 1:50-100
Immunoprecipitation: 1:10-100
Optimal dilutions to be determined by the researcher.
Positive Control:
Western Blot: Human placenta, human thyroid, and A549 lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.