Ferritin H-chain (FTH) (BSA, Azide, Glycerol Free)

Elemental iron is required for a variety of normal cellular functions and is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains, where 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3 and FTH subunit (rich in human heart) is located on chromosome 11. Ferritin is capable of storing up to 4,500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner and is also influenced by pathophysiological conditions, including inflammation and malignancy. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation and L-chains assist in core formation.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.5-1ug/ml. \nWestern Blot: 1:1000-1:5000 using ECL\nOptimal dilutions to be determined by the researcher.\n\nPositive Control for Western Blot: \nF4015-55: Ferritin H-chain, Human, Western Blot Pos. Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4015-40A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS. No BSA or azide added.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Boyd, D., et al., JBC 260: 11,755 (1985). 2. Costanzo, F., et al., EMBO J. 3: 23 (1984). 3. Chou, C.C., et al., Mol. Cell. Biol. 6: 566-573 (1986). 4. Hentze, M.W., et al., PNAS 83: 722 (1986). 5. Harrsion, P.M., et al., BBA 1275: 161 (1996). 6. Picard, V., et al., JBC 273: 15,382 (1998). 7. Rucker, P-F., et al., JBC 271: 33,352 (1996). 8. Nelson, N., et al., EMBO J. 18: 4361 (review) (1999). 9. Cairo, G., et al., Biochem. J. 352: 241-250 (2000).

Additional Info

Recognizes human Ferritin H-chain (FTH). No sequence homology with Ferritin L-chain.