FHIT, Recombinant, Human (Fragile Histidine Triad)

Cleaves A-5'-PPP-5'A to yield AMP and ADP. Possible tumor suppressor for specific tissues Alterations and deletions of the FHIT (Fragile Histidine Triad) gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach, and pancreas. In normal cells, FHIT may act as a tumor suppressor.\n\nRecombinant FHIT shows a band at 43kD on SDS-PAGE commassie blue stained gel.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Human FHIT.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4149-50B

Size

2ug

Form

Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

(same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Expression of fragile histidine triad in primary hepatocellular carcinoma and its relation with cell proliferation and apoptosis. Nan KJ, Ruan ZP, Qin HX, World J Gastroenterol 2005 Jan 14;11(2):228-31 2. Aberrations in the fragile histidine triad(FHIT) gene may be involved in lung carcinogenesis in patients with chronic pulmonary tuberculosis. Song L, Yan W, Song S, Tumour Biol 2004 Sep-Dec;25(5-6):270-5 3. Alterations of the fragile histidine triad gene in hepatitis C virus-associated hepatocellular carcinoma. Zekri AR, Bahnassy AA, El-Shehaby AM, J Gastroenterol Hepatol 2005 Jan;20(1):87-94 4. The role of human papillomavirus type 16 and the fragile histidine triad gene in the outcome of cervical neoplastic lesions. Terry G, Ho L, Cross P, Br J Cancer 2004 Dec 13;91(12):2056-62 5. [Abnormal fragile histidine triad and cervical cancer] Machida S, Saga Y, Suzuki M, Nippon Rinsho 2004 Oct;62 Suppl 10:69-72 6. Frequent silencing of fragile histidine triad gene (FHIT) in Burkitt's lymphoma is associated with aberrant hypermethylation. Hussain A, Gutierrez MI, Siraj AK, Magrath I, Genes Chromosomes Cancer 2004 Dec;41(4):321-9.