FAP (Fibroblast Activation Protein alpha; also known as seprase) is a 97kD Type II transmembrane serine protease. FAP degrades gelatin, Type I collagen and N-terminal prolines. Enzymatically active FAP is a dimer that migrates at ~170kD. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas and granulation tissue, and may play roles in tumor invasion, tissue remodeling and wound repair. The 760 amino acid (aa) human FAP contains a 735aa extracellular domain that shares 90% aa identity with mouse or rat FAP. A reported 672aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.
Applications:
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 25ug/ml. 10ul of the diluted solution labels 1-2.5x10e5 WI-38 cells in a total reaction volume (same/less than) 200ul. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as an IgG (FITC) anti-mouse.
ELISA: 2-8ug/ml. Can be used in combination with biotinylated human FAP detection antibody and recombinant human FAP as the standard.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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