Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2)

Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo.\n\nApplications: \nSuitable for use in Immunohistochemistry, Western Blot and RIA. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:1200\nImmunohistochemistry (frozen): 1:5000\nWestern Blot: 1:10,000-1:30,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4210-11C

Size

20ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Bov, Prc

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 20ul sterile ddH2O.

P Type

Pab

Purity

Serum

References

1. Gabler C, Lauer B, Einspanier A, Schams D, Einspanier R. Detection of mRNA and immunoreactive proteins for acidic and basic fibroblast growth factor and expression of the fibroblast growth factor receptors in the bovine oviduct. J Reprod Fertil. 1997;109(2):213-221. 2. Sinowatz F, Schams D, Einspanier R, Arnold G, Pfeffer M, Temmim-Baker L, Amselgruber W, Plendl J. Cellular localization of fibroblast growth factor 2 (FGF-2) in benign prostatic hyperplasia. Histol Histopathol. 2000;15(2):475 481. 3. Berisha B, Schams D, Kosmann M, Amselgruber W, Einspanier R. Expression and localisation of vascular endothelial growth factor and basic fibroblast growth factor during the final growth of bovine ovarian follicles. J Endocrinol. 2000;167(3):371-382.

Additional Info

Recognizes bovine Fibroblast Growth Factor-2. Species Crossreactivity: human, domestic ruminants, porcine FGF-2. No cross reaction with bovine FGF-1, IGF-1, IGF-2, TNFa and EGF.