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Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2)

Fibroblast Growth Factor, Basic (FGFb, BFGF, Fibroblast Growth Factor 2, FGF2, Heparin-binding Growth Factor 2, HBGF-2)

Cat no: F4210-11C

Supplier: United States Biological
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Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Applications: Suitable for use in Immunohistochemistry, Western Blot and RIA. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:1200 Immunohistochemistry (frozen): 1:5000 Western Blot: 1:10,000-1:30,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F4210-11C
Reactivities: Human, Bovine, Porcine
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 20ul
Form: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 20ul sterile ddH2O.
P type: Pab
Purity: Serum
References: 1. Gabler C, Lauer B, Einspanier A, Schams D, Einspanier R. Detection of mRNA and immunoreactive proteins for acidic and basic fibroblast growth factor and expression of the fibroblast growth factor receptors in the bovine oviduct. J Reprod Fertil. 1997;109(2):213-221. 2. Sinowatz F, Schams D, Einspanier R, Arnold G, Pfeffer M, Temmim-Baker L, Amselgruber W, Plendl J. Cellular localization of fibroblast growth factor 2 (FGF-2) in benign prostatic hyperplasia. Histol Histopathol. 2000;15(2):475 481. 3. Berisha B, Schams D, Kosmann M, Amselgruber W, Einspanier R. Expression and localisation of vascular endothelial growth factor and basic fibroblast growth factor during the final growth of bovine ovarian follicles. J Endocrinol. 2000;167(3):371-382.
Additional info: Recognizes bovine Fibroblast Growth Factor-2. Species Crossreactivity: human, domestic ruminants, porcine FGF-2. No cross reaction with bovine FGF-1, IGF-1, IGF-2, TNFa and EGF.

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