Fibronectin, Bovine

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules.1 It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when on the surface.2 Polymerization of fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), fibronectin plays an important role in normal\nmorphogenesis, including cell adhesion, migration, differentiation, and specific gene expression.\n\nActivity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture.\n\nMolecular Weight: 220kD (reduced)\n\nEndotoxin: (same/less than)1.0 EU per 1 ug of fibronectin as determined by the LAL method.\n\nApplications: ELISA, Ouchterlony, Immunoblotting. Used in vitro differentiation of mouse ES cells into neural cells. Suitable for use in mouse embryonic stem cell research. Use in Basal Medium A and as a coating agent for coverslips. This component is recommended for growth and survival of neural cell types during in vitro differentiation of mouse ES cells.\n\nUsage:\nTo coat coverslips, prepare a 1mg/ml solution. For use as a substratum for cell attachment and spreading, use at 1-5ug/cm2. To support cell spreading, add 0.5-50ug/ml in media.\n\nStorage and Stability:\nSamples are stable for up to six months at 2-8 degrees C. Product is not stable at room temperature. Avoid vortexing and excessive agitation. Aliquot into 50-100ul volumes and store at (same/less than) -20 degrees C for up to six months in a manual defrost\nfreezer. Avoid repeated freeze-thaw cycles.\n

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SPECIFICATIONS

Catalog Number

F4215-41

Size

100ug

Applications

ELISA, WB

Form

Supplied as a sterile-filtered solution in 0.4M urea, 50mM Tris-HCl (Ph 7.5) and 0.5M NaCl.\n

Purity

Purified by immunoaffinity chromatography.

References

1. Int. J. Cancer 20:1 (1977). 2. Biochem. J. 193:295 (1981). 3. Lee SH, Lumelsky N, Studer L, Auerbach JM, and McKay RD. Efficient generation of midbrain neurons from mouse embryonic stem cells. Nature Biotech. 2000:18:675.