Filaggrins are an important class of the intermediate filament-associated proteins which interact with keratin intermediate filaments (IFs) of terminally differentiating mammalian epidermis. A precursor molecule of filaggrin, profilaggrin, accumulates in the epidermis as keratohyalin granules which, in mouse, is phosphorylated and incapable of interaction with IFs. At the time of terminal differentiation, the precursor is proteolytically processed by excision of the linker to individual filaggrin molecules which are then able to interact with keratin IFs. Filaggrins exhibit wide species variations and their aberrant expression has been reported in a number of human keratinizing disorders such as parakeratosis, psoriasis and molluscum contagiosum. Filaggrin also appears to be a target molecule for rheumatoid arthritis-specific auto-antibodies in humans.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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