Filaggrin (FLG, ATOD2)

Filaggrins are intermediate filament-associated proteins that interact with keratin intermediate filaments of terminally differentiating mammalian epidermis. Filaggrins have wide species variation and are implicated in certain skin disorders and rheumatoid arthritis. This antibody will help characterize filaggrin expression in a variety of tissues.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:50-1:100 for 1 hr at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nSkin (cytoplasmic staining pattern)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4505-02C

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG1,k

References

1.Lee S C, Lee J B, Kook J P, et al.. Expression of differentiation markers during fetal skin development in humans: immunohistochemical studies on the precursor proteins forming the cornified cell envelope. Journal of Investigative Dermatology. 112(6):882-886 (1999). 2.Takahashi M, Izutani A and Tezuka T. An immunohistochemical study of abnormal keratinocyte differentiation in molluscum contagiosum. British Journal of Dermatology. 141(1):116-118 (1999). 3.Slack S L, Mannik M and Dale B A. Diagnostic value of antibodies to filaggrin in rheumatoid arthritis. The Journal of Rheumatology. 25(5):847-851 (1998). 4.Gerritsen M J, Elbers M E, de Jong E M, et al.. Recruitment of cycling epidermal cells and expression of filaggrin, involucrin and tenascin in the margin of the active psoriatic plaque, in the uninvolved skin of psoriatic patients and in the normal healthy skin. Journal of Dermatological Science. 14(3):179-188 (1997). 5.Ishida-Yamamoto A, Hashimoto Y, Manabe M, et al.. Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentia- tion. British Journal of Dermatology. 137(1):9-16 (1997). 6.Simon M, Haftek M, Sebbag M, et al.. Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis. Biochem. Journal. 317:173-177 (1996). 7.Mack J W, Steven A C and Steinert P M. The mechanism of interaction of filaggrin with intermediate filaments. Journal of Molecular Biology. 232:50-66 (1993). 8.McKinley-Grant L J, Idler W W, Bernstein I A, et al. Characterization of a cDNA clone encoding human filaggrin and localization of the gene to chromosome region 1q21. Proceedings of the National Academy of Science USA. 86:4848-4852 (1989).

Additional Info

Recognizes human Filaggrin.