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FITC anti-human CD99

FITC anti-human CD99

Cat no: 318006

Supplier: BioLegend
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The HCD99 monoclonal antibody recognizes human CD99 also known as CD99 antigen, E2 antigen, MIC2, and T-cell surface glycoprotein E2. CD99 is a type I, single chain transmembrane protein devoid of N-linked glycosylation sites that is encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, T cell leukemias and lymphomas and is absent on fetal B cells, some B cell lines, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H. The HCD99 antibody has been reported to be useful for flow cytometric detection of human CD99 and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).
Catalogue number: 318006
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100 tests
Clone: HCD99
Gene: 4267
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microl to 5 microl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microl staining volume or per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Isotype: Mouse IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: E2 antigen, MIC2, T-cell surface glycoprotein E2
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