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Products Flag (DYKDDDDK) Epitope Tag
| Catalogue number: | F4524-72F |
| Hosts: | Rabbit |
| Applications: | ELISA, Western Blot |
| Size: | 250ug |
| Form: | Suppied as liquid in PBS, pH 7.2, 0.01% sodium azide. |
| P type: | Pab |
| Isotype: | IgG |
| Purity: | Purified by affinity chromatography. |
| References: | 1. Chubet, R.G., et al., (1996), "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells", Biotechniques, 20(1): 136-141, 2. Slootstra, J.W., et al., (1997), "Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5", Mol. Divers, 2(3): 156-164, 3. Robeva, A.S., et al., (1996), "Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic proteins purification procedure", J. Biochem. Pharmacol., 51:4, 545-555, 4. Fulton, J.E., et al., (1995), "Functional analysis of avian class I (BFIV) glycoproteins by epitope tagging and mutagenesis in vitro", Eur. J. Immunol., 25:7, 2069-2076. |
| Additional info: | Recognizes the DYKDDDDK epitope tag fused to the N-terminus of targeted proteins. Does not react with fusion proteins containing the DYKDDDDK epitope tag at the C-terminus. In Western Blot against bacterial extracts, it does not crossreact with endogenous proteins. |
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