Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) (Biotin)

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR-1 (flt-1 or fms-like tyrosine kinase 1), VEGFR-2 (flk-1 or fetal liver kinase-1/KDR or Kinase insert domain containing receptor), and VEGFR-3/Flt-4. Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo. FLK-1 (Fetal-Liver Kinase 1) a putative receptor protein tyrosine kinase. FLK-1 belongs to the family of type-1 membrane protein. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. It is expressed in endothelial cells of developing embryo (1). It is also expressed at high levels in adult heart, lung, kidney, brain and skeletal muscle, but is also expressed at lower levels in most other adult tissues.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions: \nELISA: 1:5000-1:50,000; using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4565-08E

Size

50ug

Applications

ELISA

Hosts

Rat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of glycerol to 40%. Labeled with Biotin.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Oelrichs R B et al (1993) Oncogene 8, 11-18; Millauer B et al (1993) Cell 72, 835-846; Wen Y et al (1997) Gene accession # U93306; Yin LY et al (1997) Gene Acc. # AF035121; Plouet J et al (1989) EMBO J 8, 3801; Simon m et al (1998) J Am. Soc. Nephrol. Vol. 9, 1032-1044; Sait SN et al (1995) Cytogenet. Cell Genet. 70, 145; deVries C et al (1992) Science 255, 989

Additional Info

Recognizes mouse FLK13-Mb. Reacts specifically with human FLK-1/VEGFR-2/KDR. No crossreactivity with VGFR-1/FLT-1 or FLT-4.