Flumequine is a second generation quinolone antibiotic and is mainly active against Gram negative bacteria. It is used in bovine, ovine, poultry, rabbits, goats, horses and salmonidae. As all other (fluoro)quinolones, flumequine acts by inhibiting bacterial DNA-gyrase, thus disrupting its activity by interfering with the DNA annealing reaction. The MRLs for flumequine in the different matrices depend on the matrix and the species from which the matrix originates.