A novel SH-SY5Y/tGFP-DRD3 cell line has been developed through stable transfection to modulate the internalization of the Dopaminergic receptor D3 (DRD3) complex in living cells and to assay for compounds inducing tGFP-DRD3 distribution inside the cells. Fluorescent DRD3 Internalization Cell Line was obtained by transfection of an expression vector for a fusion protein of tGFP and human DRD3, as well as G418 into SH-SY5Y cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively expre Beta the tGFP-DRD3 fusion protein.Assay Details: Innoprot DRD3 Internalization Assay Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate dopaminergic receptor D3 internalization process following and quantifying the fluorescence distribution inside the cells.This highly reproducible assay allows monitoring DRD3 receptor internalization process in High Content Analysis and fluorescence microscope applications.
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