A novel U2OS/tGFP-CXCR2 cell line has been developed through stable transfection to modulate the internalization of the chemokine (C-X-C motif) receptor 2 (CXCR2) complex in living cells and to assay for compounds inducing tGFP-CXCR2 distribution inside the cells. Fluorescent CXCR2 Internalization Cell Line was obtained by transfection of an expression vector for a fusion protein of tGFP and human CXCR2, as well as puromycin into U2OS cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-CXCR2fusion protein.Assay Details: Innoprot CXCR2 Internalization Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate chemokine (C-X-C motif) receptor 2 activation and the following redistribution proce Beta inside the cells. This highly reproducible assay allows monitoring CXCR2 activation and redistribution process in High Content Analysis and fluorescence microscope applications. This internalization assay was validated with an average of Z=0.73 +/- 0.02 for High Content Screening.
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