logo
logo
Fluorescent Human Pituitary Adenylate Cyclase-Activating Polypeptide Type I Receptor (ADCYAP1R1) Internalization Assay Cell Line

Cat no: P30214

Fluorescent Human Pituitary Adenylate Cyclase-Activating Polypeptide Type I Receptor (ADCYAP1R1) Internalization Assay Cell Line

A novel U2OS/tGFP-PAC1 cell line has been developed through stable transfection to modulate the internalization of the Human Pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1) complex in living cells and to assay for compounds inducing tGFP-PAC1 redistribution inside the cells. Fluorescent PAC1 Internalization A assay cell line was obtained by stable transfection of an expression vector for a fusion protein of tGFP and human Pituitary adenylate cyclase-activating polypeptide type I receptor, as well as G418 into U2OS cells. Single cells with strong green fluorescence were selected by flow cytometry, and allowed to expand. These cells constitutively express the tGFP-PAC1 fusion protein.Assay Details: Innoprot PAC1 Internalization Cell Line has been designed to assay compounds or analyze stimuli for their ability to modulate Pituitary adenylate cyclase-activating polypeptide type I receptor activation and the following redistribution proce Beta inside the cells. This highly reproducible assay allows monitoring PAC1 receptor activation and redistribution process in High Content Analysis and fluorescence microscope applications. This internalization assay was validated with an average of Z=0.84 +/- 0.02 for High Content Screening.

Prices direct from B-Bridge International, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P30214

Size

2 vials of 3 x 10^6 cells

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...