FluoroFix Buffer

Cat no: 422101

Supplier: BioLegend

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Description
Additional Information

FluoroFix(TM) Buffer is aready-to-use buffer, specially formulated for fixation of immunofluorescence stained cells, optimized to stabilize tandem dyes. It can be used as the final resuspension of the cell pellet in immunofluorescence staining procedures. FluoroFix(TM) Buffer is provided as 100 mL.This quantity is sufficient for 200 tests.

Catalogue number:	422101
Applications:	Flow Cytometry, Immunocytochemistry
Size:	200 tests
Dilutions:	After final wash in the staining procedures (please refer to Cell Surface Immunofluorescence Staining Protocol), discard supernatant and resuspend the cell pellet in 0.5 ml/tube FluoroFix(TM) Buffer and analyzed with flow cytometry. If the samples can not be analyzed within 4 hours, resuspend the cell pellet in 0.5 ml/tube FluoroFix(TM) Buffer and incubate in the dark for 30 minutes at room temperature. Wash 1X with Biolegend Cell Staining Buffer (cat# 420201) and resuspend in 0.5ml/tube Cell Staining Buffer, cover the tubes. Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
Storage buffer:	The buffer solution should be stored at Room Temperature (RT).
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	Ambient RT
Alternative names:	Fixative, Paraformaldehyde