Folate Binding Protein, Bovine

Supplier:

United States Biological

Cat no: F5750-03

Folate Binding Protein, Bovine

Folate Binding Protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified.\n\nSource: \nFolate Binding Protein from Bovine milk.\n\nProtein content: ~2mg/ml (indirectly using the measured activity in ug/ml and the binding capacity of 8ug folic acid/mg folate binding protein.)\n \nActivity: ~14.8ug/ml (Folate binding)\n\nApplications: \nSuitable for use in ELISA. Other applications not tested\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F5750-03

Size

1mg

Applications

ELISA

Form

Supplied as a liquid in 120mM sodium phosphate, pH 7.4, 30mM sodium chloride. No preservative added.

Purity

(same/more than)95% (HPLC)

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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