Formaldehyde Assay Kit, BioAssay(TM)

Formaldehyde (methanal) is the simplest aldehyde. It is widely employed in industry for wide range of Applications:. Formaldehyde is also used as a disinfectant and is a commonly utilized tissue fixative and embalming agent. Formaldehyde is naturally present in all tissues and body fluids. Recently it has been shown that some cancer types exhibit elevated formaldehyde levels. Increased formaldehyde concentration in urine has been associated with prostate and bladder cancer. Thus, measuring formaldehyde in urine can be a very useful tool when studying cancer. \n\nThe Formaldehyde Assay Kit provides a convenient fluorimetric means to measure formaldehyde in biological samples. In the assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia. The resulting fluorescent product is then quantified fluorimetrically (lexc/em=370/470nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1.5uM formaldehyde in biological samples.\n\nKey Features:\nSafe. Non-radioactive assay.\nSensitive and accurate. As low as 1.5uM Formaldehyde can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.\nRobust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.\n\nApplications:\nFormaldehyde determination in urine and other biological samples.\n\nKit Contents:\nReagent A: 5ml Reagent B: 3ml Standard: 50ul\n10% TCA: 5ml Neutralizer: 2 x 1.5ml\nStorage conditions: store Reagent A and Reagent B at 4 degrees C. Store all other reagents at RT. Shelf life of 6 months after receipt.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nAssay Procedure:\nUse black flat-bottom plates. Prior to assay, bring all reagents to room temperature.\n1. Standards. First prepare a 35mM Formaldehyde stock by diluting 4ul of the provided Standard with 1490ul dH2O. Next, dilute 4ul of the 35mM Formaldehyde with 1396ul dH2O to make a 100uM Premix. Dilute standard as follows.\nNo Premix+H2O Formaldehyde (uM)\n1 100ul+0ul 100\n2 60ul+40ul 60\n3 30ul+70ul 30\n4 0ul+100ul 0\nTransfer 50ul standards into separate wells of the plate.\n2. Sample Preparation. Urine samples should be diluted 2-5 fold with dH2O. If urine samples contain visible particulates, then the samples should be cleared by either filtration or centrifugation (14000 rpm, 5 min). Samples high in protein (cell lysate, serum, etc.) need to be deproteinated and neutralized prior to assaying. To deproteinate, add 50ul 10% TCA per 100ul sample. Vortex and centrifuge for 5 min at 14000 rpm. Transfer 100ul of clear supernatant to a clean tube and neutralize with 25ul Neutralizer. Note: Measured dRFU

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SPECIFICATIONS

Catalog Number

F6000-03

Size

1Kit

References

1. Li, Q. et al. (2007). Development of Novel Reagent for Hantzsch Reaction for the Determination of Formaldehyde by Spectrophotometry and Fluorometry. Anal. Sci. 23: 413-7.\n2. Li, Q. et al. (2007). Flow-Injection Spectrofluorometric Determination of Trace Amounts of Formaldehyde in Water after Derivatization with Acetoacetanilide. T alanta 72: 1675-80.\n3. Fan, Q. and Dasgupta, PK (1994). Continuous Automated Determination of Atmospheric Formaldehyde at the Parts Per Trillion Level. Anl. Chem. 66: 551-6.