Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 2ug/ml. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with F9049-02D. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry (Paraffin): 1:50. Antigen retrieval with citrate, pH 6.0 is recommended.
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Positive Control:
HEK293 cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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