Home  >  Products  >  FOXO3A (Forkhead Box O3A, Forkhead in Rhabdomyosarcoma-like 1, AF6q21 Protein, DKFZp781A0677, FKHR2, FKHRL1, FKHRL1P2, MGC12739, MGC31925)
FOXO3A (Forkhead Box O3A, Forkhead in Rhabdomyosarcoma-like 1, AF6q21 Protein, DKFZp781A0677, FKHR2, FKHRL1, FKHRL1P2, MGC12739, MGC31925)

FOXO3A (Forkhead Box O3A, Forkhead in Rhabdomyosarcoma-like 1, AF6q21 Protein, DKFZp781A0677, FKHR2, FKHRL1, FKHRL1P2, MGC12739, MGC31925)

Cat no: F9049-31G

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Forkhead box O-class (FOXO) transcription factors are mammalian homologue of DAF-16, a known lifespan regulator of Caenorhabditis elegans. FOXO transcription factors, such as FOXO1, FOXO3a, and FOXO4, have been linked to the regulation of cell cycle, apoptosis, DNA repair, oxidative stress, and metabolism (1). FOXO proteins are proposed to be dual regulated by Akt-mediated phosphorylation and Skp2-mediated ubiquitination, which are usually initiated by growth factors and cytokines. FOXO proteins are also activated by stress, which induces gene expression that contributes to cell cycle arrest, implicating FOXO proteins as tumor suppressors (2). Specifically, FOXO3a (FKHR-L1) is a transcriptional activator for apoptosis in the absence of survival factors, such as in neural cell death triggered by oxidative stress. In the presence of survival factors like IGF-1, AKT1/PKB phosphorylates on Thr-32 and Ser-253. However, in the absence of survival factors, FOXO3a is dephospholyated and translocated to the nucleus inducing transcription of target genes and apoptosis (3). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2,000-5,000 Immunohistochemistry: 1:50-100 Immunocytochemistry: 1:50-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: F9049-31G
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: Forkhead (Drosophila) Homolog (rhabdomyosarcoma)-like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, FOXO2
References: 1. van der Flier A. Biochim Biophys 1538(2):99-117, 2001 2. Gorlin JB, et al. J Cell Biol 111(3):1089-105, 1990 3. Zhang W, et al. J Neurosci 18(3):914-22, 1998
Additional info: Recognizes human FOXO3a.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave