FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.\nFOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1:50. Tested on mouse thymus from an influenza-infected mouse or thymus from healthy mouse. \nWestern Blot: 1:1000-1:5000 detects a band at~47.2kD\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9050-10K

Size

100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Polanczyk MJ, et al. Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment. J Immunol. 173(4:2227-30 (2004) 2. Ochs, HD, et al. FOXP3 acts as a rheostat of the immune response. Immunol Rev. 203:156-64 (2005)

Additional Info

Recognizes human FOXP3. Species Crossreactivity: mouse

Alternative Names

MGC141961, MGC141963