FOXP3 (FOXP3, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, SCURFIN, scurfin, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked)

FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. \n\nFOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry (In paraffin embedded human tonsil, stains nuclei of some cells in the interfollicular area). Other applications not tested.\n\nRecommended Dilution: \nELISA: 1:16,000\nImmunohistochemistry: 0.3-1ug/ml\nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buf\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See F9050-10A-P. Antibody

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SPECIFICATIONS

Catalog Number

F9050-10A

Size

100ug

Applications

ELISA, IHC

Hosts

Goat

Reactivities

Hum, Can, Prc

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Chatila TA, Blaeser F, Ho N, Lederman HM, Voulgaropoulos C, Helms C, Bowcock AM. , JM2, encoding a fork head-related protein, is mutated in X-linked autoimmunity-allergic disregulation syndrome., J Clin Invest. 2000 Dec;106(12):R75-81.

Additional Info

Recognizes Human FOXP3/SCURFIN. Species crossreactivity: canine and porcine.