Fractalkine (FKN, A-152E5.2, ABCD-3, Chemokine (C-X3-C Motif) Ligand 1, C-X3-C Motif Chemokine 1, C3Xkine, CXC3, CXC3C, CX3CL1, Neurotactin, NTN, NTT, Small Inducible Cytokine D1, SCYD1)

Fractalkine, also known as neurotactin and CX3CL1, is a CX3C cytokine found in both a soluble and a membrane-bound form. The soluble form is chemotactic for T-cells and monocytes while the membrane-bound form promotes adhesion of leukocytes to endothelial cells. \n\nResearch has also demonstrated that fractalkine is able to suppress the production of nitrous oxide, IL-6 and TNF-alpha in activated microglia suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. \n\nFractalkine is widely expressed in a range of tissues including the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6069-95F

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bajetto A., et al (2001) Chemokines and their receptors in the central nervous system. Front Neuroendocrinol. 22:147-184. 2. Umehara H., et al (2001) Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1. Immunol. Cell. Biol. 79:298-302.\n3. Mizuno T., et al (2003) Production and neuroprotective functions of fractalkine in the central nervous system. Brain Res. 979:65-70. 4. Combadiere C., et al (1998) Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1. J.Biol. Chem. 273:23799-804.

Additional Info

Recognizes human Fractalkine. Species Crossreactivity: mouse.

Alternative Names

CX3C Membrane-anchored Chemokine, Small Inducible Cytokine Subfamily D Member 1