FREM1 (FRAS1-related Extracellular Matrix Protein 1, Protein QBRICK, C9orf143, C9orf145, C9orf154)

FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

146687

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Smyth I, Du X, Taylor MS, et al. The extracellular matrix gene FREM1 is essential for the normal adhesion of the embryonic epidermis. Proc. Natl. Acad. Sci. USA2004; 101:13560-5. 2. Kiyozumi D, Osada A, Sugimoto N, et al. Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle. Exp. Cell Res.2005; 306:9-23. 3. Petrou P, Makrygiannis AK, Chalepakis G, et al. The FRAS1/FREM family of extracellular matrix proteins: structure, function, and association with Fraser syndrome and the mouse bleb phenotype. Connect. Tissue Res.2008; 49:277-82. 4. Zhang X, Shephard F, Kim HB, et al. TILRR, a novel IL-1RI co-receptor, potentiates MyD88 recruitment to control Ras-dependent amplification of NF-kappaB J. Biol. Chem.2010; 285:7222-32.

Additional Info

Recognizes human FREM1. Species Crossreactivity: mouse.