FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)

FRS2 (FGFR substrate 2; also known as SNT and FRS2-Alpha) is a 70-90kD member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids (aa) in length. It contains a membrane-anchoring myristoylation signal (aa 1-6), a PTB domain that interacts with FGF and NGF receptors (aa 13-115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196-471). One splice form shows a deletion of the C-terminal four amino acids. Over aa 121-449, human FRS2 is 99% and 94% aa identical to canine and mouse FRS2, respectively.\n\nApplications: \nSuitable for use in Western Blot, Immunocytochemistry and Direct ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml with a detection limit of ~2ng/lane and 0.5ng/lane under non-reducing and reducing conditions.\nImmunocytochemistry: 3-15ug/ml, antigen retrieval is recommended.\nELISA: 0.5-1ug/ml, detection limit is ~3ng/well\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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