$FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic\nFactor-induced Tyrosine-phosphorylated Target, SNT)$

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Cat no: F7000-05D

FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic\nFactor-induced Tyrosine-phosphorylated Target, SNT)

FRS2 (FGFR substrate 2; also SNT and FSR2-a) is a 70-90kD member of the FSR family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors, and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids in length. It contains a membrane-anchoring myristoylation signal (aa1-6), a PTB domain that interacts with FGF and NGF receptors (aa13-115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa196-471). One splice form shows a deletion of the C-terminal four amino acids. Over aa121-449, human FRS2 is 99% and 94% identical to dog and mouse FRS2, respectively.\n\nApplications:\nSuitable for use in Direct ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 2.5ug/10e6 cells. Validated using A172 human glioblastoma cell line cells fixed with paraformaldehyde and permeabilized with saponin. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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