Fructose-6-Phosphate BioAssay(TM) Kit (F6P)

Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.\n\nSample Type:\nCell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products.\n\nIntended Use:\nUSBio's Fructose-6-phosphate BioAssay(TM) Kit is a fluorescence-based simple, highly sensitive and rapid means of quantifying F6P in a variety of samples.\n\nSensitivity:\n~1uM of F6P\n\nRange:\n0.01-0.5nmoles\n\nSpecificity:\nMammalian\n\nTest Principle:\nIn the assay, F6P is converted to glucose-6-phosphate which is subsequently oxidized with the generation of a fluorescent product.\n\nKit Components:\nF6P Assay Buffer: 1x25ml\nF6P Probe: 1x400ul\nF6P Enzyme Mix (lyophilized) 1 vial\nF6P Converter (lyophilized) 1 vial\nF6P Developer (lyophilized) 1 vial\nF6P Standard (10umol, lyophilized)\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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